pCDM8-ICAM-1
产品名称： pCDM8-ICAM-1
英文名称： pCDM8-ICAM-1 质粒图谱序列抗性说明书价格报价
产品编号： BiovectorPCDM8
产品价格： 0
产品产地： Biovector Inc. USA
品牌商标： Biovector, Addgene, ATCC, Invi
更新时间： null
使用范围： null
- 联系人 :
- 地址 : 北京市西直门外大街19号BioVector NTCC典型培养物保藏中心
- 邮编 : 100080
- 所在区域 : 北京
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- 邮箱 : Biovector@163.com
Product Information
|
Order ID |
Name |
Description |
|
BiovectorPCDM8 |
pCDM8-ICAM-1 |
pCDM8-ICAM-1. in E.coli, AmpR,TetR. Storage:4℃ |
Backbone vector
Gene/insert name:ICAM-1 H. sapiens (human)
Insert size:1800
Alt name:CD54
Alt name:pCD1.8
GenBank ID:NM_000201
ICAM-1 Cloning site 5:XbaI
ICAM-1 Cloning site 3:XbaI
The host strain is MC1061/P3 and the bacteria should be cultured in 12.5ug/mL ampicillin and 16ug/mL tetracycline. (supF)
Recovery
1. Obtain an LB agar plate with the appropriate antibiotic.
2. Using a sterile pipette tip, touch the bacteria growing within the punctured area of the stab culture. (A sterilized wire loop or sterile toothpick can be used in place of a sterile pipette tip.)
3. Run this tip lightly over a section of the plate, as shown in the figure, to create streak #1.
4. Using another sterile pipette tip, pass through streak #1 and spread the bacteria over a second section of the plate, to create streak #2.
5. Using a third sterile pipette tip, pass through streak #2 and spread the bacteria over the last section of the plate, to create streak #3.
6. Grow overnight in a 37 PoP C incubator (unless a different growth temperature is indicated on the plasmid datasheet).
7. In the morning, single colonies should be visible. If the bacterial growth is too dense, re-streak onto a new agar plate to obtain single colonies.
载体信息：
|
Plasmid Type: |
Mammalian Expression |
|
Promotor: |
CMV |
|
Cloning Method: |
Unknown |
|
Size: |
4356 |
|
5 Sequencing 1 Primer: |
T7 |
|
Notes: |
Start of the nucleotide sequence between the Eco-pMB1 ori and the SV40 ori. pCDM8 is a shuttle vector designed for high-level transient expression of cloned cDNA in mammalian cells. The protein encoded by the cDNA can be expressed under control of the human cytomegalovirus immediate early promoter (CMV-IE) with enhancer and the SV40 polyadenylation site. The T7 promoter is used for in vitro transcription of the cDNA insert. The small HIV (human immunodeficiency virus) fragment (29 nucleotides) between the CMV and T7 promoter sequences has an enhancer function. The presence of the polyoma and SV40 origins of replication enables the vector to replicate episomally in mammalian cells expressing large T-antigen (e.g. WOB, COS cells). The supF tRNA suppressor gene is used for selection in E. coli (MC1061[p3]). The M13 origin is useful for synthesis of single-stranded DNA upon infection with the M13 helper phage. The SspI fragment (153 bp) of the 3' UTR region of the SV40 small t-antigen is missing, but without effect on the function of the vector. |
|
Stable: |
Unspecified |
|
Constitutive: |
Constitutive |
|
Viral/Non-Viral: |
Nonviral |
载体序列：
1 ggcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt 50
51 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 100
101 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta 150
151 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 200
201 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 250
251 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 300
301 acggggggtt cgtgcacaga gcccagcttg gagcgaacga cctacaccga 350
351 actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag 400
401 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 450
451 cgcacgaggg agcttccagg gggaaacgcc tgctatcttt atgatcctgt 500
501 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 550
551 gggggcggag cctatggaaa aacgccagca acgcaagcta gcttctagct 600
601 agaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt 650
651 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat 700
701 aaatcaaaag aatagcccga gatagggttg agtgttgttc cagtttggaa 750
751 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa 800
801 ccgtctatca gggcgatggc cgcccactac gtgaaccatc acccaaatca 850
851 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 900
901 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa 950
951 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta 1000
1001 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct 1050
1051 acagggcgcg tactatggtt gctttgacga gaccgtataa cgtgctttcc 1100
1101 tcgttggaat cagagcggga gctaaacagg aggccgatta aagggatttt 1150
1151 agacaggaac ggtacgccag ctggattacc gcggtctttc tcaacgtaac 1200
1201 actttacagc ggcgcgtcat ttgatatgat gcgccccgct tcccgataag 1250
1251 ggagcaggcc agtaaaagca ttacccgtgg tggggttccc gagcggccaa 1300
1301 agggagcaga ctctaaatct gccgtcatcg acttcgaagg ttcgaatcct 1350
1351 tcccccacca ccatcacttt caaaagtccg aaagaatctg ctccctgctt 1400
1401 gtgtgttgga ggtcgctgag tagtgcgcga gtaaaattta agctacaaca 1450
1451 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg 1500
1501 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat 1550
1551 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc 1600
1601 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg 1650
1651 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 1700
1701 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggactat 1750
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